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Since the eight reactions were run on fragments of random length, the eight plus and minus reactions collectively produced DNA fragments of all possible lengths.4 The fragments in these eight reactions were separated by size (using gel electrophoresis) and then imaged with autoradiography. Gels were dried and then placed against X-ray film, allowing the radioactive DNA fragments to expose the film and appear as dark bands, which a scientist could then painstakingly translate into the DNA sequence. In 1977, Sanger and colleagues sequenced the first full DNA genome using this method: a small bacterial virus with 5,386 nucleotides in its genome, called ɸX174 or “PhiX.”
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Paramount boss David Ellison attended the State of the Union in Washington this month, as a guest of Republican Senator Lindsay Graham。业内人士推荐搜狗输入法作为进阶阅读
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